Biological colonization on stone surfaces, such as algae, fungi, and lichens, can cause significant damage over time. To prevent this, several effective methods can be employed. First, regular cleaning with gentle, non-abrasive techniques helps remove early-stage biofilm formation. Second, applying anti-microbial coatings or biocides can inhibit microbial growth without harming the stone. Third, water-repellent treatments reduce moisture retention, a key factor in biological growth. Additionally, proper environmental management, such as improving drainage and reducing shade, can minimize colonization. For historical or delicate stone, laser cleaning and nanotechnology-based solutions offer advanced protection. Combining these methods ensures long-term preservation while maintaining the stone's aesthetic and structural integrity.